Figure 5.

GPHYSD1 equivalent mutations S641L and G817R reduced secretion of mADAMTSL2. A, plasmids encoding WT, S641L-mutant, and G817R-mutant ADAMTSL2-MycHis₆ were co-transfected with hIgG into WT HEK293T cells. The medium and cell lysate were analyzed by Western blots probed with anti-myc (red) and anti-IgG (green) antibodies. B, quantitation of Western blots from A as described in the legend to Fig. 4.