Figure 6.

GPHYSD1 S641L mutation reduced O-fucosylation resulting in reduced mADAMTSL2 secretion. A, relative abundance of glycoforms of the peptide containing the O-fucose consensus sequence from TSR3 generated from WT ADAMTSL2 or S641L-mutant ADAMTSL2 TSL2-TSR3 (S641L) chymotrypsin digestion. See Figs. S2D and S3 for spectra and EICs of each peptide, respectively. Data can be found in Tables S5, S6, S9, and S10. Statistics were performed with one-way ANOVA in Prism 7, n = 3 with biological replicates. B, plasmids encoding WT or T633A-mutant ADAMTSL2 were co-transfected with hIgG into WT HEK293T cells. The medium and cell lysate were analyzed by Western blots probed with anti-myc (red) and anti-IgG (green) antibodies. C, quantitation of Western blots from B using ordinary one-way ANOVA in Prism 7, n = 3 in biological replicates. **, p < 0.01; n.s., not significant.