Figure 7.

GPHYSD1 G817R mutation did not affect N-glycosylation in TSR6 and mutation of N-glycosylation site did not restore O-fucosylation. Plasmids encoding TSP1-TSR3-MycHis₆, WT, N813Q, T815V, G817R, double mutant N813Q/T815V forms of ADAMTSL2-TSR6-MycHis₆, or an empty vector (EV) were transfected into HEK293T cells grown in the presence of 6AF as described under “Experimental procedures.” The proteins were purified from the medium and cell lysate, click reaction performed with azide-biotin, separated by SDS-PAGE, and probed with anti-myc antibody (red) or streptavidin (green).