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. 2020 Sep 6;295(46):15662–15676. doi: 10.1074/jbc.RA120.015552

Figure 2.

Figure 2.

Validation of AAV vector infusion into the murine SN and Cre recombinase expression in astrocytes. 3 weeks after AAV vector infusion, the mouse brain was perfused and coronal sections were stained with antibodies for GFAP and Cre as described under “Experimental procedures.” A, cells expressing GFP (green, 4 × magnification) depict the site of AAV vector infusion in SN regions where TH-expressing dopaminergic neurons are localized (red, TH; green, GFP; ×10 magnification). White arrows, GFP-expressing astrocytes in SN. B, YY1 is co-expressed with GFP-expressing astrocytes (top panel), and the bottom panel shows expression of YY1 (blue), TH-expressing dopaminergic neurons (red) and GFP-expressing astrocytes (green) in SN 3 weeks after AAV5-GFAP-GFP (GFAP-control) vector infusion (×20 magnification). White arrows show YY1 co-expressing with GFP. C, coronal sections immunostained with GFAP and Cre antibodies showed co-localized expression of GFAP (green) and Cre (red) in the SN region, indicating that Cre was expressed in astrocytes (×20 magnification). White arrows show Cre co-expressing with GFAP.