Figure 2.

Gut microbiota analyses in the colonic lumen of ob/ob mice. A and B, α-diversity (Shannon index, A) and β-diversity (Bray-Curtis dissimilarity matrix), B, for the luminal microbial community (n = 8-12 mice per genotype). Statistically significant difference was tested with Kruskal-Wallis test with: *, p ≤ 0.05 for α-diversity (A) and with PERMANOVA and 999 permutations for β-diversity (B). C and D, relative abundance of microbial taxa on phylum (C) and genus level (D). In the colored bar representation, microbial taxa with an abundance >1% are shown. E, heatmap showing the differentially abundant ASVs in the colonic content of ob/ob and lean mice. Heatmap is based on hierarchical clustering of the differentially abundant ASVs calculated using DESeq2 (Wald test, FDR < 0.05).