Table 3.

RT-qPCR thermal cycling conditions

Step	CDC (55)	Georgia Tech
(1) UNG incubation	25 °C, 2 min
(2) Reverse transcription	50 °C, 15 min	50 °C, 15 min
(3) RT inactivation and/or DNA polymerase activation	95 °C, 2 min	95 °C, 5 min
(4) Denaturation	95 °C, 3 s	95 °C, 15 s
(5) Annealing and extension (fluorescence collection)	55 °C, 30 s	55 °C, 30 s
Number of cycles of steps 4 and 5	45	45