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Deletion of the PiNt reduces Pif1's overexpression toxicity. A, Pif1ΔN was overexpressed in acetyltransferase mutants at 25 and 30 °C. B, Pif1ΔN was overexpressed in deacetylase mutant strains. C, comparison of the overexpression toxicity of Pif1 and Pif1ΔN in the rpd3Δ background. The graphed values represent the average of ≥3 independent experiments of technical duplicates, with error bars corresponding to the mean ± S.D. **, p < 0.01.
