Table 1.

The sgRNA sequences target PRV-Fa gE, gI, TK genes

A. Primers name	B. sgRNA sequence 5′–3′
PRV-TK-sgRNA-F	5′-caccgTGCCCGAGCCGATGGCGTGC-3′
PRV-TK-sgRNA-R	5′-aaacGCACGCCATCGGCTCGGGCAc-3′
PRV-gE-sgRNA-F	5′-caccgGCCGGCGACGATGACCTCGA-3′
PRV-gE-sgRNA-R	5′-aaacTCGAGGTCATCGTCGCCGGCc-3′
PRV-gI-sgRNA-F	5′-caccgCGCGGGGTCGTACGTGCTGC-3′
PRV-gI-sgRNA-R	5′-aaacGCAGCACGTACGACCCCGCGc-3′