Table 1.
Dark rearrangement, kA, and decay of intermediates, kD, parameters characteristic of photoactivation of membranes from WT, ∆psbO, and 27OE strains
| Strain/[Ca2+] (mM Ca2+) | kA (s−1) (t1/2 [ms]) | kD (s−1) (t1/2 [s]) | Fit quality, r |
| WT control, 2 | 2.4 (288) | 0.134 (5.17) | 0.99 |
| WT control, 10 mM Ca2+ | 2.8 (244) | 0.095 (7.29) | 0.98 |
| WT control, 40 mM Ca2+ | 1.6 (433) | 0.051 (13.5) | 0.99 |
| ∆psbO, 2 mM Ca2+ | 1.9 (364) | 0.104 (6.66) | 0.99 |
| ∆psbO, 10 mM Ca2+ | 2.2 (315) | 0.072 (9.62) | 0.99 |
| ∆psbO, 40 mM Ca2+ | 1.5 (462) | 0.049 (14.1) | 0.99 |
| 27OE, 2 mM Ca2+ | 1.5 (462) | 0.130 (5.33) | 0.98 |
| 27OE, 10 mM Ca2+ | 1.4 (495) | 0.085 (8.15) | 0.99 |
| 27OE, 40 mM Ca2+ | 1.8 (385) | 0.027 (25.6) | 0.99 |
Data were fit to Eq. 2 to estimate the dark rearrangement constant, kA, and the decay of photoactivation intermediates “B and C," kD (see Fig. 1 for model). Error bars represent standard deviation n ≧ 3.