Fig. 3.

The CITED2 peptide inhibits FGF2-induced neovascularization in the in vivo Matrigel plug assay. (A) Representative photos of Matrigel plugs that were removed from the s.c. space 5 d postinjection. (Scale bars, 1 cm.) (B) Cryosectioned Matrigel plugs were stained with the endothelial cell marker CD31 (green) and DAPI (blue). (Scale bars, 200 μm.) (C) Cell sorting from Matrigel plugs. Matrigel plugs were digested and stained with CD31 and CD45. The CD31 positive/CD45 negative fraction was considered to be endothelial cells. The percentage of cells in the CD31 positive/CD45 negative fraction is indicated in the Lower Right quadrant of the graphs in red. (D) Quantification of endothelial cells in Matrigel plugs (n = 8). P values were calculated using an unpaired two-tailed t test, *P < 0.05, N.S., not significant. The mean and SEM are shown in red.