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. 2020 Oct 23;117(45):28275–28286. doi: 10.1073/pnas.2008921117

Fig. 5.

Fig. 5.

GPR56 is required for efficient hemostasis and platelet plug formation. Mouse tail injury bleeding assay results are reported (A) as times to final bleeding cessation and (B) in detail to show instances of temporary bleeding, stoppage, and resumption. (C–E) Mouse cremaster arteriole laser-induced injury assay showing representative platelet plug formation (green platelets) and fibrin accumulation (red). The still images accompany Movie S1. Quantification of mean fluorescence intensity (MFI) of platelet accumulation or fibrin accumulation. Error bars are the mean ± SEM (n = 30 and 28 injuries among three Gpr56+/+ or Gpr56−/− mice, respectively). (F) Mouse tail injury bleeding assay of wild-type mice injected with vehicle or an equivalent volume of purified GPR56 rNTF protein (5 mg/kg). (G) Mouse cremaster arteriole laser-induced injury assay showing representative platelet plug formation and fibrin accumulation in wild-type mice injected with vehicle or purified GPR56 rNTF protein (5 mg/kg). The still images accompany Movie S2. (H and I) Quantification of MFI of platelet accumulation and fibrin accumulation. Error bars are the mean ± SEM (n = 13 injuries among two mice injected with vehicle and n = 21 injuries among three mice injected with GPR56 rNTF). *P < 0.05, **P < 0.005; ns, nonsignificant.