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. 2020 Oct 23;117(45):28275–28286. doi: 10.1073/pnas.2008921117

Fig. 7.

Fig. 7.

GPR56 is required for shear force-dependent platelet adhesion to immobilized collagen. Gpr56+/+, Gpr56+/−, and Gpr56−/− mouse blood was incubated with DiOC6 to stain platelets fluorescently green and was flowed through small bore chambers coated with immobilized collagen at (A and B) a high shear rate to mimic arterial blood flow-induced shear forces or (C and D) a low shear rate to mimic typical venous blood flow-induced shear force. Time-dependent platelet adhesion to collagen was monitored by video microscopy. The representative still images accompany Movies S4 (arterial shear) and S5 (venous shear). Mean fluorescence intensity (MFI) of the adhered and aggregated platelet signals. (E and F) Human blood was treated with DiOC6 and supplemented with vehicle or 250 nM GPR56 rNTF. The blood was flowed over immobilized collagen at a high shear rate (1,800 s−1), and platelet adhesion was monitored by time-lapse video microscopy. The representative still images accompany Movie S6. MFI of the adhered platelet signals. Error bars are the mean ± SEM (n = 5 flow trials for each condition). *P < 0.05. ns, nonsignificant.