Fig. 7.

Detection of cell type-specific changes in glycan shield composition and dynamics with cryo-EM. (A) Mean intensity at each glycan BMA residue for the BG505_293F, BG505_293S, and BG505_CHO cryo-EM maps along with the percent change from BG505_293F. (B–D) BG505_293F − BG505_293S (B), BG505_CHO − BG505_293S (C), and BG505_293F − BG505_CHO (D) difference maps at two intensity thresholds. All difference maps were multiplied by a soft mask around the RM20A3 Fabs and then smoothed with a 2-SD Gaussian filter. (E–H) Flyouts of the regions outlined by dashed red boxes in B–D.