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. 2020 Oct 29;117(45):28307–28315. doi: 10.1073/pnas.2016997117

Fig. 5.

Fig. 5.

APPL2 interacts with RacGAP1 in a glucose-dependent manner in INS-1E cells. HEK293 cells (A, B) or INS-1E cells (C) were cotransfected with plasmids encoding FLAG-tagged APPL2 and HA-tagged RacGAP1 for 48 h. INS-1E cells were stimulated with glucose (16.7 mM) for the indicated time points. The transfected cells were subjected to IP using an antibody against HA tag (A) or FLAG tag (B and C). (D) INS-1E cells stimulated with glucose (16.7 mM) for the indicated time points were subjected to IP using an anti-RacGAP1 antibody or nonspecific IgG as a negative control, followed by immunoblotting analysis as indicated. (E) Schematic of FLAG-tagged full-length (FL) APPL2 and its truncated mutants. (F) HEK293 cells were transfected with indicated APPL2 plasmids and HA-RacGAP1, followed by IP and immunoblotting analysis. Representative images were shown from three independent experiments.