Skip to main content
. 2020 Nov 16;10(7):e228. doi: 10.1002/ctm2.228

FIGURE 8.

FIGURE 8

LPS stimulation for 6 h enhanced the transcriptional activation of STING via c‐Myc. A, Promoter sequence of STING in Homo sapiens. B, Mutation analysis of candidate transcription factor for binding sites in promoter region of STING in HEK 293 cells. C, Chromatin immunoprecipitation assay of c‐Myc and STING promoter with or without c‐Myc overexpression in HEK 293 cells. D, Relative promoter activity of STING with or without c‐Myc overexpression in HEK 293 cells. E and F, Western blots and statistical results for phosphorylated STING and STING after c‐Myc was upregulated or inhibited. G, Western blots and statistical results for c‐Myc with or without LPS stimulation in peritoneal macrophages. H, Chromatin immunoprecipitation assay of c‐Myc and STING promoter. I, Relative promoter activity of STING. J, Relative mRNA levels of STING, NLRP3, IL‐1β, and IL‐18 (n = 6, *P<.05 vs indicated group, NS means no significance)