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Proposed modifications of Cas12a for improved editing efficiency in plants. (i) Improved dCas12a activators and repressors for modifying gene expression, (ii) High efficiency gene targeting through homology repair mechanisms, (iii) Nickases, (iv) Chemically modified and structurally engineered crRNA, (v) Base editors for point mutations and indel insertions, (vi) Increase genome editing efficiency at low temperature, (vii) PAM-flexible Cas12a variants.
