TABLE 2.

List of modified and native Cas12a nucleases with their flexible “PAM” specificities.

CRISPR/Cas12a system	Modification in native CRISPR/Cas12a	PAM specificity	Amino acids Size	References
FnCas12a	Native	TTV,TTTV,KYTV	1300	Zetsche et al., 2015
FnCas12a-RR	N607R/K671R	TYCV, TCTV	1300	Toth et al., 2018; Zhong et al., 2018; Tóth et al., 2020
FnCas12a-RVR	N607R/K613V/N617R	TWTV	1300	Toth et al., 2018; Zhong et al., 2018; Tóth et al., 2020
FnCas12a-RVRR	N607R/K613V/N617R/K671R	TYCV, TCTV, TWTV	1300	Tóth et al., 2020
AsCas12a	Native	TTTV	1307	Zetsche et al., 2015; Kim et al., 2017; Tang et al., 2017; Bernabé-Orts et al., 2019
AsCas12a-RR	S542R/K607R	TYCV,CCCC	1307	Gao et al., 2017
AsCas12a-RVR	S542R/K548V/N552R	TATV	1307	Gao et al., 2017
AsCas12a-RVRR	S542R/K548V/N552R/K607R	TYCV,CCCC,TATV	1307	Tóth et al., 2020
LbCas12a	Native	TTTV	1228	Zetsche et al., 2015; Kim et al., 2017; Tang et al., 2017; Bernabé-Orts et al., 2019; Li et al., 2019b
LbCas12a-RR	G532R/K595R	TYCV,CCCC	1228	Gao et al., 2017; Li et al., 2018a; Zhong et al., 2018; Tóth et al., 2020
LbCas12a-RVR	G532R/K538V/Y542R	TATV	1228	Gao et al., 2017; Li et al., 2018a; Zhong et al., 2018; Tóth et al., 2020
LbCas12a-RVRR	G532R/K538V/Y542R/K595R	TYCV,CCCC,TATV	1228	Tóth et al., 2020
MbCas12a	Native	TTV, TTTV	1373	Zetsche et al., 2015
MbCas12a-RR	N576R/K637R	TYCV, TCTV	1373	Toth et al., 2018
MbCas12a-RVR	N576R/K582V/N586R	TWTV	1373	Toth et al., 2018
MbCas12a-RVRR	N576R/K582V/N586R/K637R	TYCV, TCTV, TWTV	1373	Tóth et al., 2020
enAsCas12a	E174R/S542R/K548R	VTTV,TTTT,TTCN,TATV	1307	Kleinstiver et al., 2019
impLbCas12a	D156R, G532R, K538V, Y542R, k595R	TTTV,TCCV, CCCV, TATC, TACV	1228	Tóth et al., 2020