Table 7. Age of blood donors and proliferative response.

Study	Number ofblood donors	Age (y.)blood donor	Gender	Cell type	Optimal PRP concentrationcell proliferation(Vol/Vol and plt/ μ L or fold)
Haynesworth et al. (2002)	No information	No information	No information	Fibroblasts	10% of 1.6 × 106(5 folds over baseline)
Graziani et al. (2006)	3	24-29	Yes	Fibroblasts	33.3% of 2.5x ≈ 0.570 × 106
Rughetti et al. (2008)	8	No information	No information	Endothelial cells	1.25 × 106(PRP/media ratio unclear)
Anitua et al. (2009)	2	“Young”	No information	Fibroblasts (skin and synovium)	20% of 0.767 ± 95 × 106and0.404 ± 39 × 106
Hsu, Kuo & Tseng (2009)	20	29.3 ± 6.5	No information	Fibroblasts (PDL)	5% of 1.124 × 106of5% (Vol/Vol)
Mishra et al. (2009)	No	No information	No information	Fibroblasts (skin)	10% of 1 × 106
Jo et al. (2012)	9	52.7 ± 19.2	No information	Tenocytes	10% of 4.00 × 106(CaCl2)and10% of 8.00 × 106(CaCl2+ thrombin)
Mazzocca et al. (2012)	8	31.6 ± 10.9	No information	Tenocytes	10% of PRPLP: 382.0+/-111.6 × 10310% of PRPDS: 472.6+/-224.2 × 103
Chen et al. (2012)	10 Pooled	No information	No information	Dental pulp stem cells	5% of 1.0 × 1065% of 1.124 × 106of5% (Vol/Vol)
Wang et al. (2012)	3	(24, 26, 41)Mean: 28.3	No information	Tenocytes	10% of 1.5-1.9 × 106(4x)
Sadoghi et al. (2013)	No	No information	No information	Unclear	5-fold ≈ 1.25 × 106(PRP/media ratio unclear)
Amable et al. (2014)	3 pr. pool	No information	No information	HMSC	10% of 1 × 106
Giusti et al. (2014)	3	(52, 30, 50) Mean: 44	M: 2 F: 1	Tenocytes	0.5 × 106(PRP/media ratio unclear)
Tavassoli-Hojjati et al. (2016)	1	No information	No information	Fibroblasts (periodontal ligament)	5% of 1.194 × 106
Berger et al. (2019)	14 Pooled	27 ± 5 (Young group)	M: 4, F: 2 M: 2, F: 6	Fibroblasts (Achilles, patellar, and palmaris)	20% of0.875 × 106(Young group)20% of3.5 × 106(Old group)
		63 ± 11 (Old group)
Wang et al. (2012)	8 Individual	39.3 ± 5.8	M: 4 F: 4	HMSC (bone marrow)	10% of 1.5 to 3.0 × 106