Table 2. Performance comparison of DNA extraction methods.
| CTAB | Genomic-tip | Mollusc DNA | TIANamp Marine Animals DNA | Sbeadex livestock | |
|---|---|---|---|---|---|
| Time | |||||
| -Total assay time | 2 h 30 min | 7-8 h | 17 h (overnight lysis step) | 3 h | 4 h |
| Hands-on time | 1 h 30 min | 1 h 30 min | 1 h | 45 min | 2 h |
| Costa | Inexpensive (2 USD/rnx/100 mg tissue) | Expensive (27.4 USD/rnx/100 mg tissue) | Expensive (8.2 USD/rnx/50 mg tissue) | Inexpensive (2.5 USD/rnx/30 mg tissue) | Expensive (7.2 USD/rnx/50 mg tissue) |
| Yield | Low yield (8.81 ng DNA/mg tissue) | Highest yield (96.38 ng DNA/mg tissue) | Low yield (13.15 ng DNA/mg tissue) | Lowest yield (8.25 ng DNA/mg tissue) | Moderate yield (56.42 ng DNA/mg tissue) |
| Quality | |||||
| − Integrity | HMW observed in >48.5 kb | HMW observed in >48.5 kb | Smearing of DNA observed in <20 kb | Smearing of DNA observed in <20 kb | HMW observed in 48.8 kb and smearing of DNA observed in <48.5 kb |
| − Purity | Poor | Good | Good | Poor | Poor |
Notes.
a
Cost indicated here was converted from the local currency to USD. It may vary in other countries depending on costs from local distributors.