Table 2. Characteristics of cytokine primers developed in the study for ddPCR for IL-4 and IFNγ in N. cinerea.

Gene name	Primer sequences (5′->3′)	Probe fluorophore	Annealing/Extension (°C, s)	Optimal primer/probe concentration (nM)	Amplicon size (Bp)
IL-4	F: TCACCTCCCAACTGATTCCAA		60, 20	400	132
	R: ACGAGTCGTTTCTCGCTGT		60, 20	400
	P: GCACTCACCAGCACCTTTGTCCA	FAM	60, 20	100
IFNy	F: AGCTGATTCGAATTCCCGTGA		58, 20	400	95
	R: TCTGACTCCTTTTCCGCTTCC		58, 20	400
	P: TGCAGGTCCAGCGCAAAGCGATA	FAM	58, 20	100