Table 3. Nucleotide identity of qPCR products for IL-4, IL-6, IFNγ, IL-10 and TNFα represented by the best match in BLASTn search (http://www.ncbi.nlm.nih.gov/BLAST/) in the four target species.
| Target gene | qPCR product | % Best match | Accession Number |
|---|---|---|---|
| IL-4 | Neophoca cinerea | 98 | Eumetopias jubatus XM_028103636.1 |
| Canis familiaris | 95 | Canis familiaris EF095771.1 | |
| IL-6 | Neophoca cinerea | 100 | Zalophus californianus XM_027574842.1 |
| Canis familiaris | 88 | Canis familiaris AF349534.1 | |
| IFNγ | Neophoca cinerea | 98 | Enhydra lutris XM_022495107.1 |
| Canis familiaris | 97 | Canis lupus XM_025476664.1 | |
| IL-10 | Neophoca cinerea | 100 | Eumetopias jubatus XM_028120121.1 |
| Canis familiaris | 95 | Canis familiaris EU426968.1 | |
| Bos taurus | 95 | Bos indicus KX013148.1 | |
| Ovis aries | 97 | Equus caballus XM_023640225.1 | |
| TNFα | Neophoca cinerea | 90 | Eumetopias jubatus XM_028120686.1 |
| Canis familiaris | 87 | Vulpes vulpes KM892854.1 | |
| Bos taurus | 95 | Bos taurus NM_173966.3 | |
| Ovis aries | 86 | Ovis aries EF446377.1 |