Table 4. qPCR: efficiency (E%) and linearity (R²) for IL-4, IL-6, IFNγ, IL-10, TNFα and GAPDH in their respective targeted species.

Target gene	Species	Slope	R2	E (%)
IL-4	Neophoca cinerea	−3.267	0.987	102
	Canis familiaris	−3.244	0.990	103
IL-6	Neophoca cinerea	−3.293	0.985	101
	Canis familiaris	−3.351	0.999	99
IFNγ	Neophoca cinerea	−3.229	0.999	104
	Canis familiaris	−3.225	0.996	103
IL-10	Neophoca cinerea	−3.132	0.996	95
	Canis familiaris	−3.319	0.998	100
	Bos taurus	−3.083	0.994	111
	Ovis aries	−3.167	0.996	107
TNFα	Neophoca cinerea	−3.374	0.989	98
	Canis familiaris	−3.271	0.936	102
	Bos taurus	−3.624	0.998	89
	Ovis aries	−3.684	0.999	87
GAPDH	Neophoca cinerea	−3.385	0.992	106
	Canis familiaris	−3.581	0.998	87
	Bos taurus	−3.288	0.979	101
	Ovis aries	−3.371	0.998	98