Table 6. qPCR and ddPCR validation parameters for IL-4 and IFNγ primer-probe pairs in Neophoca cinerea.
| qPCR | ddPCR | ||||||
|---|---|---|---|---|---|---|---|
| Target gene | E (%) | R2 | LoD | LoQ | Copies in pooled samples | LoD | LoQ |
| (Copy numbers) | (Copy numbers) | ||||||
| IL-4 | 101 | 0.996 | 6 | 199 | 13 | 4.19 | 12.68 |
| IFNγ | 101 | 0.997 | 5 | 119 | 69 | 3.48 | 10.54 |
Note:
qPCR efficiency (E%), linearity (R2), Limit of Detection (LoD) and Limit of Quantification (LoQ). ddPCR Quanta-Soft Analysis ProTM™ software copy number per reaction for Neophoca cinerea pooled samples and LoD and LoQ of the assays.