FIGURE 2.

Total RNA editing in mtRNA, KREH2-IPs, and RESC6-IPs: (A) Site-by-site, and (B) cumulative analysis in the RPS12 ORF sequence. Total editing at each site is scored as the percentage of RNA-seq reads showing any editing event, either correct or incorrect, at that site. T-str and ES positions, including those forming the start and stop codons are indicated. Each 10 in the T-str sequence is indicated (+). In B, The RPS12 ORF sequence is divided into two halves (T-str positions 31–88 and 89–151, divided by a vertical line). T-str position 51 “ES54” marked by an empty arrowhead (A,B) indicates the first position in a cluster of ESs with increased total editing in the ORF 5′ half in RESC6-IPs. Values and error bars reflect the mean of n = 2 +SD independent biological replicates. Blue bars represent frequent gRNAs for RPS12 in an extensive gRNA transcriptome study (Koslowsky et al. 2013). Differently colored vertical bars represent the boundaries of each editing block. A sliding window analysis was performed. Three windows centered at positions T-str 43, 65, and 94, respectively, are shown. P-values <0.0005 (***), <0.005 (**), <0.05 (*), and >0.05 (NS) comparing RESC6-IP to mtRNA (top tier) or KREH2-IP to mtRNA (bottom tier) were annotated in panel A.