Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec;26(12):1919–1934. doi: 10.1261/rna.076760.120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Ramachandran et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

FIGURE 4. — Analysis of SNORDs identified in this study. (A) Distribution of SNORDs guiding methylations in rRNA using box D, box D′, or both. (B) Base pair interaction between the antisense element in the SNORD and the rRNA target in relation to the distance from box D (or D′). (C) Sequence logo of the box D, D′, C, and C′ of SNORDs guiding methylations in rRNA generated by WebLogo (Crooks et al. 2004). (D, upper left) sequence comparison of human and zebrafish early- and late-rRNA containing a conserved methylation at LSU-2824 with indications of identical (.), deleted (-), and methylated (m) nucleotides. (Lower left) Human and zebrafish SNORD34 and the interaction with the target sequence surrounding LSU-2824. Nucleotides that differ between the two are labeled in red. (Right) 2D structures of a conserved LSU rRNA region between human and zebrafish highlighting methylations in H47, H48, H60, and H61. Methylation at LSU-2824 is in red and the zebrafish-specific methylation in blue. (E, upper) sequence comparison of human and zebrafish early- and late-rRNA highlighting LSU-Cm3916 only found in late-rRNA. (Lower) Drawings of the SNORD base-pairings with early- and late-rRNA, respectively. The presence of a mismatch (x) with early-rRNA may prevent pairing and methylation. (Hsa) Homo sapiens, (Zbf) zebrafish.