FIGURE 2.

ADAR1 augments the DICER cleavage reaction rate for NFκB1 Alu dsRNAs and increases Alu siRNA production. (A) Secondary structure of NFκB1 intronic Alu dsRNA. (B,C) The time course analysis of DICER cleavage. The DICER reaction was done at 37°C with 0.15 nM of NFκB1 Alu dsRNA and 1.5 nM of DICER alone or DICER complexes for various times. The reaction products were fractionated by 10% Urea-PAGE. The Alu siRNA cleavage efficiencies were determined at the 30 min time point. Data are shown as mean ± SD. (n = 3 technical replicates). (*) P < 0.05; (***) P < 0.001; n.s. not significant by two-tailed Student's t-test.