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. 2020 Dec;26(12):2031–2043. doi: 10.1261/rna.077354.120

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© 2020 Immer et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 4. — Lpp1663 has a preference for U-rich single-stranded RNAs. The ITC thermograms show Lpp1663 titrated with an oligo U₆, A₆, C₆, or a 6mer G-rich RNA. Lpp1663 has the highest affinity to oligo U₆ (top, left). In comparison, the affinity to oligo A₆ is ∼2.5-fold lower (top, right). Lpp1663 does not bind oligo C₆ or the G-rich 6mer single-stranded RNA (bottom) in ITC-experiments.