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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Dev Biol. 2020 Sep 6;467(1-2):108–117. doi: 10.1016/j.ydbio.2020.08.008

Figure 2: Individual DIF-FRAC elution profiles show distinct changes consistent with RNAse sensitivity.

Figure 2:

(A) Table of DIF-FRAC calculated Z-scores for ribosomal proteins and selected others. Values highlighted in green and yellow are considered high confidence. Values in red are of borderline confidence and should be evaluated with prior knowledge. (B-D) Individual profiles for ribosomal subunits. X-axis represents SEC fractions from larger molecular weight to smaller. Y-axis represents observed abundance in MS by unique peptide spectral matches normalized to the highest value for that protein. (E) Ribosomal subunit, Rpl35a, had a Z-score below the cutoff (A), but its elution profile shows consistent behavior with other ribosomal subunits. (F) Known RNA-binding protein, Nucleolin, shows shift in molecular weight. (G) Known RNA-binding protein, Puf60, shows increased observed abundance. (H-I) Profiles of RNA-binding proteins with known roles in Xenopus development. (J-K) Elution profiles of negative controls do not change.