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. 2020 Nov 12;219(12):e202002154. doi: 10.1083/jcb.202002154

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© 2020 Liu et al.

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Figure S1. — Fluid dropping from a fixed distance has a mechanical stimulation effect comparable to that of FFSS. CEF cells were infected with high-titer recombinant RCAS(A) retroviruses containing Cx50, Cx46, or P88S or coinfected retroviruses containing P88S and Cx50 or Cx46. Mechanical stimulation was applied by dropping the medium from a fixed distance. LY/RD dye uptake was conducted after mechanical stimulation. The percentage of cells with LY dye uptake (excluding cells that took up both LY and RD-dextran) was quantified. Each data point in the graph represents an individual quantified image. Data are presented as mean ± SEM. **, P < 0.01; ***, P < 0.001.