Figure S2.

Integrin α6 is expressed in cortical lens, primary chick lens culture, and CEF cells. (A) Crude membrane extracts were prepared from whole chick lens, CEF cells, and differentiated primary chick lens culture containing lentoids and immunoblotted with anti-integrin α6 antibody and β-actin antibody. (B) Crude membrane extracts were prepared from whole chick lens (W) or isolated E18 chick lens portions containing cortical fibers (CF) or nuclear fibers (NF). Integrin α6 was detected in the cortical lens fibers, but not in the central, nuclear lens fiber. As no actin was expressed in nuclear lens, Ponceau S staining was used to show that a comparable amount of protein was loaded on SDS-PAGE.