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. Author manuscript; available in PMC: 2020 Nov 16.
Published in final edited form as: Nat Neurosci. 2020 Feb 17;23(3):386–397. doi: 10.1038/s41593-020-0590-1

Extended Data Fig. 2. Live tracking of CGG RAN translation in neuronal dendrites.

Extended Data Fig. 2

A) Left: Venus with and without (ΔAUG) an AUG initiation codon serve as a positive and negative translation control. Right: Venus fluorescent proteins with the AUG deleted were inserted after the 5’ leader of FMR1, in the +1 (FMRpolyG) reading frame to serve as a reporter for +1CGG RAN. B) Live imaging of mature rat hippocampal neurons expressing indicated Venus reporters (green) and mCherry (red). C) Quantification of Venus reporter signals with 32 repeats (n=11) or 90 repeats (n=14) (p=0.0002). D) Single molecule imaging of CGG RAN translation in distal neuronal processes expressing indicated +1CGG RAN-Venus reporters after photo bleaching. Green dots represent individual translation events. E) Quantification of CGG RAN events in processes (n=3; p=0.2355). Panels C, E: Two sided Student’s t-test. n.s.=not significant, ***p<0.001. Graphs are mean +/− S.E.M.