Table I.
Target | Primer sequence | Tm optimal (°C) |
---|---|---|
Mst1 | Forward: AGCACCGATTTACGCCAGAA | 54°C |
Reverse: CCGTACCTTTGGTCTCACCC | ||
Mst2 | Forward: TCCCTCTTTCCGCAAACCTC | 63.3°C |
Reverse: GATTTGGAGGGGGTCCGAAG | ||
SAV1 | Forward: GCGGGGAAAGTTTACGGGAT | 66.5°C |
Reverse: CAGTCGCTGGTCAGTTCCTT | ||
LATS1 | Forward: CGACGCTCACGAACGATCAG | 54°C |
Reverse: ATGTAGCCCACACGAAGGAC | ||
LATS2 | Forward: CAATGTAGCGAATGTCCCACTT | 57°C |
Reverse: TGAAGATTATCACTCTCTCCAGGG | ||
CTGF |
Forward: AAACTGGAACGGTGAAGGTG Reverse: CTCAAGTTGGGGGACAAAAA |
59.9°C |
Primer name, sequence and optimal melting temperature (Tm optimal).