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. 2020 Nov 16;2020(4):hoaa048. doi: 10.1093/hropen/hoaa048

Table I.

Target gene primer details.

Target Primer sequence Tm optimal (°C)
Mst1 Forward: AGCACCGATTTACGCCAGAA 54°C
Reverse: CCGTACCTTTGGTCTCACCC
Mst2 Forward: TCCCTCTTTCCGCAAACCTC 63.3°C
Reverse: GATTTGGAGGGGGTCCGAAG
SAV1 Forward: GCGGGGAAAGTTTACGGGAT 66.5°C
Reverse: CAGTCGCTGGTCAGTTCCTT
LATS1 Forward: CGACGCTCACGAACGATCAG 54°C
Reverse: ATGTAGCCCACACGAAGGAC
LATS2 Forward: CAATGTAGCGAATGTCCCACTT 57°C
Reverse: TGAAGATTATCACTCTCTCCAGGG
CTGF

Forward: AAACTGGAACGGTGAAGGTG

 

Reverse: CTCAAGTTGGGGGACAAAAA

59.9°C

Primer name, sequence and optimal melting temperature (Tm optimal).