Figure 3.

Bright field and immunofluorescent staining of lungs containing metastases derived from either murine FAP-transfected (right) or non-transfected (left) B16 melanoma cells at day 21 after injection of tumor cells. Pigmented B16 cells can be recognized in the bright field (top row). Murine FAP in cyan blue, cell nuclei in blue (DAPI). In the top row cut ends of cryo-section tissue blocks are depicted so that metastases can be localized in the corresponding stained cryo-sections below (middle row). The close-up images (bottom row) show normal lung tissue and parts of metastases. Red arrows indicate borders of metastases