Figure 1. Profilin is present in centrosomes throughout the cell cycle.

Fluorescence microscopy revealed the co-localization of profilin and γ-tubulin. (A) Ab staining of profilin and γ-Tb in B16 cells after a brief pretreatment with 0.1% Triton X-100 before fixation (see text). High-resolution confocal microscopy (AiryScan). (B) B16 cells expressing citrine-profilin (a, d, g; green) were captured at different cell cycle stages. Cells were permeabilized with 10 μM digitonin, fixed, and stained for γ-tubulin (b, e, h; red). Superposition of images in (c, f, i). The cell in (d, e, f) was captured at an early G2-stage of the cell cycle and the one in (g, h, i) at late G2/prophase. Micrographs were obtained by spinning disk microscopy. Scale bars (A) and (B): 10 μm.