Figure 5.

Fluorescence quenching of apo-hTf emission spectra with increasing amounts of Cp₂VCl₂ at296 K (A), 303 K (B), 310 K (C) and 317 K (D). A progressive decrease in the fluorescence intensity and a remarkable red shift was observed with addition of Cp₂VCl₂. The protein apo-hTf was excited at 280 nm, and its emission spectra was recorded in the range of 290 nm to 500 nm. Inset: Stern-Volmer plots of F₀/F versus [Q].