Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Ambystoma mexicanum) | d/d axolotl | Ambystoma genetic stock center | RRID:AGSC_101L | |
| Commercial assay, kit | 5-Ethynyl-2’-deoxyuridine (EdU) |
clickchemistrytools.com | Cat# 1149 | Monomer analog |
| Commercial assay, kit | 5-Ethynyl Uridine (5-EU) | clickchemistrytools.com | Cat# 1261 | Monomer analog |
| Commercial assay, kit | L-Azidohomoalanine (AHA) | clickchemistrytools.com | Cat# 1066 | Monomer analog |
| Commercial assay, kit | N-azidoacetylglucosamine-tetraacylated (Ac4GlcNAz) | clickchemistrytools.com | Cat# 1085 | Monomer analog |
| Commercial assay, kit | AFDye 488 Azide | clickchemistrytools.com | Cat# 1275 | Fluorescent azide |
| Commercial assay, kit | AFDye 594 Azide | clickchemistrytools.com | Cat# 1295 | Fluorescent azide |
| Commercial assay, kit | AFDye 594 Alkyne | clickchemistrytools.com | Cat# 1297 | Fluorescent alkyne |
| Chemical compound, drug | 2,2′-Thiodiethanol (TDE) | Sigma | Cat# 166782 | Clearing agent |
| Chemical compound, drug | L(+)-Ascorbic acid sodium salt | Thermo | Cat# AC352680050 | Click-it cocktail component |
| Chemical compound, drug | Copper(II) sulfate pentahydrate, 98+% | Thermo | Cat# AC423615000 | Click-it cocktail component |
| Chemical compound, drug | SlowFade Gold Antifade Mountant | Thermo | Cat# S36936 | Mountant |