Table 1. Summary table of expression pattern and wit-responsiveness for BMP-LA containing DNA fragments tested in vivo.
The first column indicates the name for each of the cloned BMP-LA containing DNA fragments; these were sorted based on intensity and pattern of reporter expression. The second column provides information on reporter expression in the VNC, (more plus signs indicate higher intensity), and the final column provides details on expression pattern. The third and fourth columns indicate fragments that exhibited pMad and reporter co-expression, as well as the ones that were shown to be wit-responsive. Bolded letters indicate the enhancer fragments that were further tested for wit-responsiveness. The expression pattern was assessed in wandering third instar larvae.
| DNA fragment | VNC expression | Reporter/pMad stain overlap | Wit responsive | VNC expression details |
|---|---|---|---|---|
| CM5 | +++ | √ | √ | neurons and glia |
| CM1 | +++ | √ | √ | medial and lateral neurons |
| CM4 | +++ | √ | √ | medial and lateral neurons |
| CM3 | +++ | √ | √ | medial and lateral neurons |
| CM2 | ++ | √ | √ | medial and lateral neurons |
| CM7 | + | √ | √ | sparse |
| CM6 | + | √ | √ | sparse |
| CM8 | +++ | √ | - | medial neurons |
| CM9 | ++ | √ | - | lateral neurons |
| CM10 | + | √ | - | sparse |
| CM11 | ++ | - | - | neurons and glia |
| CM12 | + | - | - | sparse |
| CM13 | + | - | - | sparse |
| CM14 | + | - | - | sparse |
| CM15 | + | - | - | sparse |
| CM16 | + | - | - | sparse |
| CM17 | + | - | - | low intensity |
| CM18 | + | - | - | low intensity |
| CM19 | + | - | - | low intensity |
| CM20 | - | - | - | none |
| CM21 | - | - | - | none |
| CM22 | - | - | - | none |
| CM23 | - | - | - | none |
| CM24 | - | - | - | none |