Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (L. pneumophila Lp02) | Lp02∆lem27 | This paper | N/A | A lem27 deletion mutant of strain Lp02 |
| Bacterial strain, strain background (L. pneumophila Lp02) | Lp02∆lem27∆lotA | This paper | N/A | A lem27 and lotA deletion mutant of strain Lp02 |
| Bacterial strain, strain background (L. pneumophila Lp02) | Lp02∆sidC∆sdcA | Hsu et al., 2014 | N/A | A sidC and sdcA deletion mutant of strain Lp02 |
| Strain, strain background (L. pneumophila) | Lp02∆sidC∆sdcA(pZL199) | This paper | N/A | Strain Lp02∆sidC∆sdcA expressing SidC |
| Strain, strain background (L. pneumophila) | Lp02∆sidC∆sdcA(pZL199 and pLem27) | This paper | N/A | Strain Lp02∆sidC∆sdcA expressing SidC and Lem27 |
| Strain, strain background (L. pneumophila) | Lp02∆sidC∆sdcA(pZL199 and pLotA) | This paper | N/A | Strain Lp02∆sidC∆sdcA expressing SidC and LotA |
| Cell line (Human) | HEK293T | ATCC | CRL-1573 | |
| Cell line (Human) | Hela | ATCC | CCL-2 | |
| Cell line (Mouse) | RAW264.7 | ATCC | TIB:71 | |
| Cell line (Human) | U937 | ATCC | CRL-1593.2 | |
| Cell line (Mouse) | MLE | ATCC | CRL-2110 | |
| Antibody | Mouse monoclonal ANTI-FLAG antibody M2 | Sigma | Cat. #: F1804 | WB (1: 3000) IF (1: 200) |
| Antibody | Mouse monoclonal ANTI-GFP antibody | Sigma | Cat. #: SAB5300167 | WB (1:5000) |
| Antibody | Mouse monoclonal ANTI-His antibody | Sigma | Cat. #: H1029 | WB (1: 10,000) |
| Antibody | Mouse monoclonal Anti-HA antibody | Santa Cruz | Cat. #: sc-7392 | WB (1: 1000) |
| Antibody | Rabbit polyclonal Anti-ICDH antibody | Xu et al., 2010 | N/A | WB (1: 20,000) |
| Antibody | Mouse monoclonal Anti-tubulin antibody | DSHB | E7 | WB (1: 10,000) |
| Antibody | Rabbit polyclonal Anti-Lem27 antibodies | This paper | N/A | WB (1:500) |
| Antibody | Rabbit polyclonal Anti-SidC antibodies | Luo and Isberg, 2004 | N/A | WB (1:10000) |
| Antibody | Rabbit polyclonal Anti- L. pneumophila antibodies | Xu et al., 2010 | N/A | IF (1:10,000) |
| Antibody | Mouse monoclonal Anti-FKI antibody | Enzo Life Science | Prod. No. BML-PW8805 | IF (1:1,000) |
| Peptide, recombinant protein | 3XFlag Peptide | Sigma-Aldrich | Cat. #: F4799 | |
| Commercial assay or kit | Quikchange kit | Agilent | Cat. #: 600670 |
|
| Commercial assay or kit | TransStart Fast Pfu DNA Polymerase | TransGen, Beijing, China | Cat. #: AP221-03 | |
| Chemical compound, drug | Ubiquitin-Propargylamine(Ub-PA) | Boston Biochem | Cat. #: U-214 | |
| Chemical compound, drug | AQUApure Di-Ub Chains (K6-linked) Protein, CF | Boston Biochem | Cat. #: UC-11B-025 | |
| Chemical compound, drug | AQUApure Di-Ub Chains (K11-linked) Protein, CF | Boston Biochem | Cat. #: UC-40B-025 | |
| Chemical compound, drug | Recombinant Human Di-Ub/Ub2 WT Chains (K27-linked), CF | Boston Biochem | Cat. #: UC-61B-025 | |
| Chemical compound, drug | AQUApure Di-Ub Chains (K29-linked) Protein, CF | Boston Biochem | Cat. #: UC-81B-025 | |
| Chemical compound, drug | AQUApure Di-Ub Chains (K33-linked) Protein, CF | Boston Biochem | Cat. #: UC-101B-025 | |
| Chemical compound, drug | AQUApure Di-Ub Chains (K48-linked) Protein, CF | Boston Biochem | Cat. #: UC-200B-025 | |
| Chemical compound, drug | AQUApure Di-Ub Chains (K63-linked) Protein, CF | Boston Biochem | Cat. #: UC-300B-025 | |
| Chemical compound, drug | Antibiotic G418 | Sigma-Aldrich | Cat. #: G8168 |
|
| Software, algorithm | HHpred | Soding et al., 2005 | ||
| Software, algorithm | Adobe Photoshop CS3 Extended | Adobe | ||
| Software, algorithm | Graphpad | graphpad.com | ||
| Other | Anti-HA Magnetic Beads | MedChemExpress (MCE) | Cat. #: HY-K0201 | |
| Other | Anti-flag M2 affinity gel | Sigma | Cat. #: A2220 |