Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain (Mus musculus) | C57BL/6J Mice | https://www.jax.org/strain/000664 | 000664 | 6–12 weeks old |
| Commercial assay or kit | Nuclei isolation Kit: Nuclei EZ Prep | Sigma-Aldrich | NUC101-1KT | |
| Antibody | E-cadherin Monoclonal antibody (ECCD-2) | Thermofisher Scientific | 13–1900 | IF (1:250) |
| Antibody | Human/mouse NCAM-1/CD56 Polyclonal antibody | R and D Systems | AF2408-SP | IF (1:25) |
| Antibody | Anti-EpCAM Polyclonal antibody | Abcam | ab71916 | IF (1:1000) |
| Antibody | Anti-Stra6 Polyclonal antibody | Sigma-Aldrich | ABN1662 | IF (1:100) |
| Antibody | Anti-Igf1r Polyclonal antibody | R and D Systems | AF305 | IF (1:50 w/ antigen retrieval) |
| Antibody | Anti-Slco2a1 Polyclonal antibody | Atlas Antibodies | HPA013742 | IF (1:25 w/ antigen retrieval) |
| Antibody | Anti-Lepr Polyclonal antibody | R and D Systems | AF497 | IF (1:200) |
| Antibody | Anti-Pcdh12 Polyclonal antibody | Abcam | ab113720 | IF (1:25 w/ antigen retrieval) |
| Antibody | Anti-Podxl Polyclonal antibody | R and D Systems | AF1556 | IF (1:25) |
| Antibody | Anti-Pecam1 Polyclonal antibody | Abcam | ab23864 | IF (1:50) |
| Antibody | Anti-Vegfa Polyclonal antibody | Abcam | ab51745 | IF (1:50) |
| Antibody | Anti-Met Polyclonal antibody | R and D Systems | AF276 | IF (1:100) |
| Antibody | Anti-Egfr Polyclonal antibody | R and D Systems | AF1280 | IF (1:100) |
| Antibody | Anti-Gata1 Monoclonal antibody | Cell Signaling | 3535 | IF (1:100) |
| Antibody | Anti-Hbegf Polyclonal antibody | R and D Systems | AF8239 | IF (1:20) |
| Software, algorithm | R | https://www.r-project.org/ | ||
| Software, algorithm | ImageJ | ImageJ (http://imagej.nih.gov/ij/) | ||
| Software, algorithm | Seurat (3.1.3) | https://satijalab.org/seurat/ | ||
| Software, algorithm | cellranger (3.0.2) | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/feature-bc | ||
| Software, algorithm | CellPhoneDB | https://www.cellphonedb.org/ | ||
| Software, algorithm | SCENIC | https://github.com/aertslab/SCENIC | ||
| Software, algorithm | ClusterProfiler | https://guangchuangyu.github.io/software/clusterProfiler/ | ||
| Software, algorithm | Slingshot | https://github.com/kstreet13/slingshot | ||
| Software, algorithm | scVelo | https://github.com/theislab/scvelo | ||
| Software, algorithm | FlowJo | https://www.flowjo.com |