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Immunocytochemical staining of Ki-67, mucin 5AC (MUC5AC), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) in infected middle ear mucosa explants on postexplantation day 3. Immunocytochemistry shows decreased expression in the explants with higher concentrations of amniotic membrane extract (AME; ×400, scale bar=50 μm).
