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Knockdown of ASK1 attenuated ERS, ROS production, and inflammatory factors. (a) The production of fibroid- and ERS-related proteins was inhibited by ASK1 siRNA. (b) Immunofluorescence assays were performed to measure α-SMA expression in HSCs. (c) ROS production was inhibited by ASK1 siRNA. (d–f) The production of inflammatory factors IL-18 (d), IL-1β (e), and TNF-α (f) was blocked by ASK1 siRNA. ∗p < 0.05 and ∗∗p < 0.01 vs. blank; #p < 0.05 vs. Ang II.
