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. 2020 Nov 12;15:8875–8892. doi: 10.2147/IJN.S270208

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© 2020 Lu et al.

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Characterization and cytological evaluation of PPTA/pVAXI-En. (A and B) SEM images and agarose gel electrophoresis of PEI/pVAXI-En, PPA/pVAXI-En, PPT/pVAXI-En and PPTA/pVAXI-En. (C) Cytotoxicity of PEI, PPA, PPT and PPTA at various concentrations against HUVECs. ** and *** Indicate p<0.01 and p<0.001 versus the PEI group, respectively. (D) Fluorescence images of the intracellular distribution of PEI/FITC-pVAXI-En, PPA/FITC-pVAXI-En, PPT/FITC-pVAXI-En and PPTA/FITC-pVAXI-En in HUVECs at 2 h (left) and 4 h (right) after the beginning of transfection. Green, FITC-labeled pVAXI-En plasmid; blue, cell nuclei stained with Hoechst 33342; red, lysosomes stained with LysoTracker Red. Bars represent 10 μm.