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. 2020 Nov 12;15:8875–8892. doi: 10.2147/IJN.S270208

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© 2020 Lu et al.

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The gene transfection efficiency of PPTA in vitro and vivo. (A) Fluorescent images of HUVECs transfected with PEI/pEGFP-N1, PPA/pEGFP-N1, PPT/pEGFP-N1 or PPTA/pEGFP-N1. Bars represent 200 μm. (B) Flow cytometric histogram of the ﬂuorescence intensity of HUVECs. (C) Quantitative evaluation of EGFP expression by flow cytometry in HUVECs. * Indicates p<0.05 when compared with the PEI group. ^# indicates p<0.05 when compared with the PPA group. (D) In vivo transfection efficiency. Fluorescence images of brain sections separated from intracranial U87 glioma-bearing nude mice transfected with PEI/pEGFP-N1, PPA/pEGFP-N1, PPT/pEGFP-N1 or PPTA/pEGFP-N1. Green, EGFP protein; blue, cell nuclei stained with DAPI. Bars represent 50 μm.