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. 2020 Nov 3;14:581830. doi: 10.3389/fnsys.2020.581830

Figure 7.

Figure 7

Stimulating the LPO reduces foot-shock avoidance. (A) In vivo optogenetics setup: we injected either hSyn-ChR2 (ChR2) or hSyn-mCherry (mCherry) in the LPO and implanted a fiber overlaying the injection site. (B) Timeline for RTPT. Note that pairing remains consistent across training and the optically paired side becomes dual-paired with electricity. (C) RTPT electricity procedure. (D) Time spent in the initially paired side across RTPT decreased across days as the initially paired side was paired with electricity (day effect: F(17,255) = 30.34, P < 0.001). The mCherry group decreased to a greater degree than the ChR2 group (day × group interaction: F(17,255) = 5.55, P < 0.001). (E) The number of initially paired intervals greater than 3 s across RTPT decreased across days as the initially paired side was paired with electricity (day effect: F(17,255) = 26.71, P < 0.001). The mCherry group decreased to a greater degree than the ChR2 group (day × group interaction: F(17,255) = 4.06, P < 0.001). In (D,E), faded lines depict values from individual rats; points and error bars depict mean and SEM, respectively (HSD ChR2 vs. mCherry, *P < 0.05).