Schematic representation of experimental set up for Controls, along with single dose and fractionated radiation for PC3 and DU145 cells (A). Heat map of differentially expressed genes in prostate carcinoma cells (PC3and DU145) after SD (5 Gy, 10 Gy) and MF (0.5 Gy and 1 Gy x 10) radiation at 24 hours (B) Heat map of differentially expressed genes in HCAEC at 6 and 24 hours after an SD (10Gy) and 6 and 24 hours after the final dose of MF (2Gyx5) irradiation (C). Yellow to red, upregulated; blue, downregulated genes. Inflection point kinetics of immune genes for interleukin 29 (IL29), interferon induced transmembrane protein 1 (IFITM1), and interferon induced protein 44 (IFI44) in multifractionated treated PC3 and DU145 cells as assessed by real time RT-PCR. Treatment was 1 Gy, 3x/day with at least 6 hours between fractions (D).