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. Author manuscript; available in PMC: 2021 Mar 14.
Published in final edited form as: Nat Chem Biol. 2020 Sep 14;16(12):1434–1439. doi: 10.1038/s41589-020-0641-7

Extended Data Figure 8. Characterization of aY-modified PercevalHR (ATP sensor).

Extended Data Figure 8.

(a) Fluorescence excitation (dash line) and emission (solid line) profiles for PercevalHR before (cyan) and after (dark cyan) addition of 1 mM ATP, and aY-PercevalHR before (magenta) and after (red) addition of 1 mM ATP. (b) Representative images of HeLa cells expressing aY- PercevalHR in response to 10 mM 2-deoxy-D-glucose (2-DG). Scale bar: 20 μm. (c) Quantitative traces for randomly selected five single cells (gray dots) and their average (red line). Intensities are normalized to the values at t = 0 s. The time points for addition of chemicals are shown as arrows. These experiments were repeated three times with similar results using independent biological samples.