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. Author manuscript; available in PMC: 2021 Mar 14.
Published in final edited form as: Nat Chem Biol. 2020 Sep 14;16(12):1434–1439. doi: 10.1038/s41589-020-0641-7

Table 2.

Photophysical properties of the tested genetically encoded biosensors and their aY-modified variants.

Biosensor Analytical target λex a (nm) λem b (nm) Dynamic Range c (%) εd (mM−1 •cm−1) φ e
− + − +
G-GECO1 34 Ca2+ 497 512 1,983 4.7 68.1 0.26 0.38
aY-G-GECO1 Ca2+ 528 594 1,566 7.7 79.9 0.14 0.22
ZnGreen1 35 Zn2+ 474 500 2,531 24.8 1.3 0.48 0.35
aY-ZnGreen1 Zn2+ 520 585 915 34.7 4.8 0.28 0.20
iGluSnFR 36 glutamate 488 510 257 9.6 32.6 0.58 0.60
aY-iGluSnFR glutamate 528 600 270 12.2 42.7 0.46 0.49
iGABASnFR 37 GABA 502 513 143 NDf NDf NDf NDf
aY-GABASnFR GABA 541 602 92 NDf NDf NDf NDf
dLight1.2 38 dopamine 496 513 194 NDf NDf NDf NDf
aY-dLight1.2 dopamine 545 603 117 NDf NDf NDf NDf
SoNar 39 NAD+/NADH 497 512 257 [2,532]g 7.6 23.0 0.44 0.51
aY-SoNar NAD+/NADH 544 604 426 [2,656]g 6.5 28.6 0.29 0.35
iNap1 40 NADPH 497 513 82 [273]g 22.6 13.1 0.50 0.43
aY-iNap1 NADPH 545 604 614 [11,735]g 27.6 4.4 0.41 0.36
PercevalHR 41 ATP 498 513 155 [364]g 14.6 35.6 0.28 0.28
aY-PercevalHR ATP 545 604 117 [650]g 18.4 40.5 0.21 0.21
iATPSnFR1.1 42 ATP 493 513 106 22.6 43.9 0.36 0.37
aY-iATPSnFR1.1 ATP 541 608 75 29.1 50.9 0.28 0.28
a

wavelength of the excitation peak near 490 and 540 nm for green and aY-modified, red fluorescent biosensors, respectively.

b

wavelength of the emission peak.

c

unless otherwise specified, dynamic range is defined as the intensiometric change (ΔF/Fmin) around emission peaks in the presence of 480 and 540 nm excitation for green and aY-modified, red fluorescent biosensors, respectively.

d

extinction coefficient in the presence (+) or absence (−) of the corresponding analyte. For SoNar and aY-SoNar, presented are values with NAD+ (+) or NADH (−).

e

quantum yield.

f

not determined.

g

in square brackets are dynamic ranges (Rmax/Rmin) of the sensors operated in the excitation-ratiometric mode; defined as the ratio of the intensity ratios at two excitation peak wavelengths.