Figure 4. Activity of ICAM1-ADC on cell lines in vitro.

(A-D) ICAM1-ADC selectively inhibits cell proliferation of myeloma cell lines RPMI8226, JJN3, INA6 and AMO1 in vitro. Percent cell viability after 72 h treatment shown. EC₅₀ values were estimated by curve-fitting and are as follow: RPMI8226 1.9 nM, JJN3 2.2 nM, INA6 3.6 nM, and AMO1 4.2 nM. (E) The MFI of ICAM1 expression correlated inversely with cell line EC50 for sensitivity to ICAM1-ADC. (F) Tested against RPMI8226 for comparison after 96 h incubation (EC₅₀ = 0.6 nM), ICAM1-ADC has relatively little effect on cells with low ICAM1 expression, including normal T cells, HUVEC and Hs27 cells. Ctrl ADC – nonbinding isotype control ADC.