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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Mitochondrion. 2020 Sep 24;55:100–110. doi: 10.1016/j.mito.2020.09.006

Figure 4. Status of exosome-derived proteins in the post-exosome SEC fractions and following trypsin digestion.

Figure 4.

(A) Western blots for CD-9, TSG101, FGF21, and citrate synthase proteins in exosome and post-exosome fractions generated from SH-SY5Y cell culture medium (CCM). Amido black staining illustrates the degree of overall protein loading. Note the amido black blots were cut horizontally during the course of data generation, thereby generating upper and lower sections; we present the amido black data in a way that clearly acknowledges this. (B) Western blots for CD-9, TSG101, TFAM, and VDAC1 proteins in the exosome and post-exosome fractions generated from plasma, with and without exposure of those fractions to trypsin. Amido black staining illustrates the degree of overall protein loading. Note the amido black blots were cut horizontally during the course of data generation, thereby generating upper and lower sections; we present the amido black data in a way that clearly acknowledges this. Data shown are representative of two or three experiments.