Figure 4.

STS regulates AR signaling. VCaP cells were treated with SI-1 or SI-2. RNA was collected and sent for RNAseq. A. Heatmap depicting alterations in AR responsive genes. B. GSEA analysis of AR pathway. C. Heatmap depicting the effects of SI-1 or SI-2 on genes kown to be upregulated by ARv7. D. C4–2B or VCaP cells were treated with SI-1 or SI-2 for 3 days, total RNA was extracted and KLK3, NKX3–1 or FKBP5 mRNA levels were analyzed by qRT-PCR. E. C4–2B or VCaP cells were transiently transfected with PSA luciferase plasmid and then treated with different concentrations of SI-1 or SI-2 overnight. PSA luciferase activity was determined. F. VCaP cells were transiently transfected with STS siRNA, total RNA was extracted and KLK3, NKX3–1 and FKBP5 mRNA levels were analyzed by qRT-PCR.G. VCaP cells were transiently transfected with STS siRNA. PSA luciferase activity was determined. * p<0.05.